Katılma tarihi: 13 May 2022

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Vital Source Converter Serial 43




*). The serum and bile samples were stored at −80 °C until analysis. Serum chemical parameters {#Sec13} ------------------------- The serum biochemical parameters were measured by routine laboratory methods in the Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan. The serum samples were analyzed for TG, cholesterol, T-CHO, ALB, AST, ALT, ALP, LDH, GGT, CK, CK-MB, and creatinine by using an automated analyzer (Cobas Integra 400 plus; Roche Diagnostics, Indianapolis, IN, USA). Serum lipids were analyzed using a Boehringer Mannheim Hitachi 7600--020 Automatic Analyzer (Hitachi Medical Co., Tokyo, Japan). Briefly, free fatty acid (FFA) was measured by using a nonesterified fatty acid determination kit (Wako Pure Chemical Industries, Ltd., Osaka, Japan). The TG level was measured using a TG quantification kit (BioVision Research Products, Mountain View, CA, USA). The HDL-C level was determined by using an enzymatic cholesterol esterification method (Cholesterol E-test; Wako Pure Chemical Industries, Ltd.) and the LDL-C level was determined by using the Friedewald formula^[@CR24]^. All chemicals were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany) and the kits were purchased from Roche Diagnostics (Mannheim, Germany). Urine chemical parameters {#Sec14} A total of 1 g of faecal sample was homogenized in 10 mL of 0.9% NaCl. The sample was centrifuged at 3000 rpm for 10 min, and the supernatant was recovered. The pH of the supernatant was measured using a pH meter (Model TPS-1M, Shanghai Tope Instruments Co., Ltd., Shanghai, China). Then, the pH value of the supernatant was adjusted to 4.0. Urine samples were obtained from an overnight fasted animal and stored at −80 °C. The total urine volume was measured by a water-filled measuring cylinder. To adjust for differences in urine volume, urinary specific gravity was determined by using an automatic specific gravity meter (SGU-111, ZhengDa Instruments Co., Ltd., Shanghai, China). In




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Vital Source Converter Serial 43

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